Project

Part:BBa_K2475002:Design

Designed by: Shawn Cody Receno   Group: iGEM17_Waterloo   (2017-10-25)


Sup 35 Prion Domain fused to Yellow Fluorescent Protein (PrD-YFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 709
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Considered what fluorescent proteins would work well with FRET experiments to show our proof of principle actually functions.


Source

Gene fusion of Sup35 Prion domain and Yellow Fluorescent protein.

References